Part III: Proteomics
The Protein Universe
Proteomics is the large-scale study of proteins — their expression levels, modifications, interactions, and structures. Because proteins are the primary functional molecules of the cell, proteomics bridges the gap between genomic information and biological function. This part covers protein separation techniques, mass spectrometry-based identification and quantification, post-translational modification analysis, and protein interaction networks.
>20,000
Human Proteins
>400
Known PTM Types
10⁶
Dynamic Range
Topics in This Part
9. Protein Separation & 2D-PAGE
SDS-PAGE, isoelectric focusing, two-dimensional gel electrophoresis, chromatographic methods, and sample preparation
10. Mass Spectrometry Proteomics
Ionization methods (ESI, MALDI), mass analyzers, tandem MS, peptide fragmentation, and database searching algorithms
11. Quantitative & PTM Proteomics
Label-free and labeled quantification (SILAC, TMT, iTRAQ), phosphoproteomics, glycoproteomics, and ubiquitomics
12. Interactomics & Structural Proteomics
Yeast two-hybrid, co-IP/MS, cross-linking MS, cryo-EM, AlphaFold, and protein interaction network analysis