Sequencing Technologies

Genomics, Proteomics, Transcriptomics & Metabolomics · Part 1

247 KB10 sections4 key equationsLaTeX typeset
Download PDFRead Online (Free)

Table of Contents

  1. 1.Sanger Sequencing (Chain Termination Method)
  2. 2.Next-Generation Sequencing: Illumina Platform
  3. 3.Third-Generation Sequencing
  4. 4.Quality Scores & Error Profiles
  5. 5.Sequencing Depth, Coverage & the Lander-Waterman Model
  6. 6.Principle of Chain Termination
  7. 7.Reaction Components
  8. 8.Sanger Sequencing Characteristics
  9. 9.Library Preparation
  10. 10.Bridge Amplification & Cluster Generation

Key Equations

$$Q = -10 \log_{10}(P_e)$$
$$P_e = 10^{-Q/10}$$
$$C = \frac{N \times L}{G}$$
$$C = \frac{10^9 \times 150}{3.2 \times 10^9} = \frac{1.5 \times 10^{11}}{3.2 \times 10^9} \approx 47\times$$

Equations are rendered with MathJax in the PDF with professional LaTeX typesetting.

Course Context

This PDF is part of the Genomics, Proteomics, Transcriptomics & Metabolomics course on CoursesHub.World. Study genomics, proteomics, transcriptomics and metabolomics with 20 free chapters covering DNA sequencing, RNA-Seq, mass spectrometry, metabolic profiling, multi-omics integration, and precision medi...

← Genome Organization
Gene Expression →

Get Instant Access to 461+ PDF Study Guides

Professional LaTeX-typeset PDFs with complete derivations, worked examples, and beautiful equation rendering. Download any PDF, anytime. Cancel anytime.

$5
per month
All courses included
Save 17%
$50
per year
Best value
Subscribe Now
Secure payment via StripeCancel anytimeInstant access