Mass Spectrometry

Genomics, Proteomics, Transcriptomics & Metabolomics · Part 3

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Table of Contents

  1. 1.10.1 Fundamentals of Mass Spectrometry
  2. 2.10.2 Ionization Methods for Biomolecules
  3. 3.10.3 Mass Analyzers
  4. 4.10.4 Tandem Mass Spectrometry & Fragmentation
  5. 5.10.5 Bottom-Up & Top-Down Proteomics Workflows
  6. 6.10.6 Database Searching & Statistical Validation
  7. 7.Key Performance Metrics
  8. 8.Fragmentation Methods
  9. 9.Peptide Mass Fingerprinting (PMF)
  10. 10.Bottom-Up (Shotgun) Proteomics

Key Equations

$$\frac{m}{z} = \frac{M + z \cdot m_{H^+}}{z}$$
$$R = \frac{m}{\Delta m_{50\%}}$$
$$a_u = \frac{8eU}{m\omega^2 r_0^2}, \quad q_u = \frac{4eV}{m\omega^2 r_0^2}$$
$$\text{Score} = -10 \cdot \log_{10}(P)$$

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Course Context

This PDF is part of the Genomics, Proteomics, Transcriptomics & Metabolomics course on CoursesHub.World. Study genomics, proteomics, transcriptomics and metabolomics with 20 free chapters covering DNA sequencing, RNA-Seq, mass spectrometry, metabolic profiling, multi-omics integration, and precision medi...

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