Protein Separation

Genomics, Proteomics, Transcriptomics & Metabolomics · Part 3

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Table of Contents

  1. 1.9.1 Protein Extraction & Sample Preparation
  2. 2.9.2 SDS-PAGE: Principles & Practice
  3. 3.9.3 Two-Dimensional Gel Electrophoresis (2D-PAGE)
  4. 4.9.4 Western Blotting & Immunodetection
  5. 5.9.5 Chromatographic Protein Separation
  6. 6.9.6 Protein Staining & Quantification Assays
  7. 7.Cell Lysis Strategies
  8. 8.Subcellular Fractionation
  9. 9.The Discontinuous Buffer System
  10. 10.The Ferguson Plot

Key Equations

$$R_f = \frac{d_{\text{protein}}}{d_{\text{front}}}$$
$$\log(\mu) = \log(\mu_0) - K_R \cdot T$$
$$\%T = \frac{(a + b)}{V} \times 100 \qquad \%C = \frac{b}{a + b} \times 100$$
$$\text{pH} = \text{p}K_a + \log\left(\frac{[\text{A}^-]}{[\text{HA}]}\right)$$

Equations are rendered with MathJax in the PDF with professional LaTeX typesetting.

Course Context

This PDF is part of the Genomics, Proteomics, Transcriptomics & Metabolomics course on CoursesHub.World. Study genomics, proteomics, transcriptomics and metabolomics with 20 free chapters covering DNA sequencing, RNA-Seq, mass spectrometry, metabolic profiling, multi-omics integration, and precision medi...

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