Quantitative Proteomics

Genomics, Proteomics, Transcriptomics & Metabolomics · Part 3

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Table of Contents

  1. 1.11.1 Label-Free Quantification
  2. 2.11.2 Isotope Labeling Strategies
  3. 3.11.3 Data Acquisition Strategies: DDA vs. DIA
  4. 4.11.4 Post-Translational Modification (PTM) Analysis
  5. 5.11.5 Targeted Proteomics: SRM, MRM & PRM
  6. 6.Spectral Counting
  7. 7.Ion Intensity-Based Quantification
  8. 8.SILAC (Metabolic Labeling)
  9. 9.Chemical Labeling: TMT & iTRAQ
  10. 10.Phosphoproteomics Enrichment

Key Equations

$$\text{NSAF}_k = \frac{\text{SpC}_k / L_k}{\displaystyle\sum_{i=1}^{N} (\text{SpC}_i / L_i)}$$
$$SI_N = \frac{\displaystyle\sum_{j=1}^{p_k} \text{Fragments}_j}{L_k \cdot \displaystyle\sum_{i=1}^{N}\left(\frac{\displaystyle\sum_{j=1}^{p_i} \text{Fragments}_j}{L_i}\right)}$$
$$\text{iBAQ} = \frac{\displaystyle\sum_{i=1}^{n} I_i}{N_{\text{theoretical}}}$$
$$\text{CV} = \frac{\sigma}{\mu} \times 100\%$$

Equations are rendered with MathJax in the PDF with professional LaTeX typesetting.

Course Context

This PDF is part of the Genomics, Proteomics, Transcriptomics & Metabolomics course on CoursesHub.World. Study genomics, proteomics, transcriptomics and metabolomics with 20 free chapters covering DNA sequencing, RNA-Seq, mass spectrometry, metabolic profiling, multi-omics integration, and precision medi...

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